56 Single-cell proteomic chip for profiling intracellular signaling pathways in single tumor cells.
http://www.pnas.org/content/109/2/419.abstract
55 Optical recording of action potentials in mammalian neurons using a microbial rhodopsin.
http://www.nature.com/nmeth/journal/v9/n1/abs/nmeth.1782.html
54 Global profiling of dynamic protein palmitoylation.
http://www.nature.com/nmeth/journal/v9/n1/abs/nmeth.1769.html
53 Decoding cell lineage from acquired mutations using arbitrary deep sequencing.
http://www.nature.com/nmeth/journal/v9/n1/abs/nmeth.1781.html
52 Unique molecular identifiers (UMIs) associate distinct sequences with every DNA or RNA molecule and can be counted after amplification to quantify molecules in the original sample. Using UMIs, the authors obtain a digital karyotype of an individual with Down's syndrome and quantify mRNA in Drosophila melanogaster cells.
http://www.nature.com/nmeth/journal/v9/n1/abs/nmeth.1778.html
51 Conjugation of triplet-state quenchers to the small organic cyanine fluorophore, Cy5, increases photostability without affecting its spectral characteristics. This allows longer fluorescence imaging with a concomitant reduction in blinking both in vitro and in living cells.
http://www.nature.com/nmeth/journal/v9/n1/abs/nmeth.1774.html
50 Biodegradable poly(amine-co-ester) terpolymers for targeted gene delivery.
http://www.nature.com/nmat/journal/v11/n1/abs/nmat3187.html
49 The rational design of a synthetic polymer nanoparticle that neutralizes a toxic peptide in vivo.
http://www.pnas.org/content/109/1/33
48 Inhibition of specific gene expressions by protein-mediated mRNA interference.
http://www.nature.com/ncomms/journal/v3/n1/full/ncomms1621.html
47 Three-colour single-molecule fluorescent in situ hybridization is used by van Oudenaarden and colleagues to show overlapping expression of intestinal stem-cell markers during homeostasis, ageing and regeneration. This approach can help identify putative stem cells in tissues and tumours, and can guide functional studies.
http://www.nature.com/ncb/journal/v14/n1/abs/ncb2384.html
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