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Biotechnology

Biotechnology

Articles:

29  Brakemann et al. present a reversibly photoswitchable fluorescent protein, called Dreiklang, that can be turned on and off at wavelengths distinct from those used for imaging. They show that the protein is advantageous for studying protein dynamics in living cells and for super-resolution imaging.
http://www.nature.com/nbt/journal/v29/n10/abs/nbt.1952.html

28  Protein delivery using engineered virus-like particles.
http://www.pnas.org/content/108/41/16998.abstract

27  Selective fluorescent probes for live-cell monitoring of sulphide.
http://www.nature.com/ncomms/journal/v2/n10/full/ncomms1506.html

26  An Expanded Palette of Genetically Encoded Ca2+ Indicators. Zhao Y. et al. (2011). Science 333: 1888-1891.
http://www.sciencemag.org/content/333/6051/1888.abstract

25  Delivery of siRNA and other macromolecules into skin and cells using a peptide enhancer. Hsu T. and Mitragotri S. (2011). PNAS 108:15816-15821.
http://www.pnas.org/content/108/38/15816.abstract

24  Sensitive detection of cytokines in 1-μl serum samples using Proseek.
http://www.nature.com/nmeth/journal/v8/n9/abs/nmeth.f.348.html

23  A fluorescent protein that can be photoswitched with visible light from orange to far-red is presented. The photoconverted form has the most red-shifted excitation peak of all GFP-like fluorescent proteins to date and should be useful for many imaging applications. Subach O et al. (2011). Nat Methods 8: 771-777.
http://www.nature.com/nmeth/journal/v8/n9/abs/nmeth.1664.html

22  Intravenous delivery of a multi-mechanistic cancer-targeted oncolytic poxvirus in humans. Breitbach CJ et al. (2011). Nature 477: 99-102.
http://www.nature.com/nature/journal/v477/n7362/full/nature10358.html

21  Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors. ban H. et al. (2011). pNAS 108: 14234-14239.
http://www.pnas.org/content/108/34/14234.abstract

20  High-throughput microfluidic single-cell RT-qPCR. White AK. et al. (2011). PNAS 108: 13999-14004.
http://www.pnas.org/content/108/34/13999.abstract

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