23 A fluorescent protein that can be photoswitched with visible light from orange to far-red is presented. The photoconverted form has the most red-shifted excitation peak of all GFP-like fluorescent proteins to date and should be useful for many imaging applications. Subach O et al. (2011). Nat Methods 8: 771-777.
http://www.nature.com/nmeth/journal/v8/n9/abs/nmeth.1664.html
22 Intravenous delivery of a multi-mechanistic cancer-targeted oncolytic poxvirus in humans. Breitbach CJ et al. (2011). Nature 477: 99-102.
http://www.nature.com/nature/journal/v477/n7362/full/nature10358.html
21 Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors. ban H. et al. (2011). pNAS 108: 14234-14239.
http://www.pnas.org/content/108/34/14234.abstract
20 High-throughput microfluidic single-cell RT-qPCR.
White AK. et al. (2011). PNAS 108: 13999-14004.
http://www.pnas.org/content/108/34/13999.abstract
19 High-level recombinant protein expression in transgenic plants by using a double-inducible viral vector. Werner S. et al. (2011). PNAS 108: 14061-14066.
http://www.pnas.org/content/108/34/14061.abstract
18 Long-term in vivo glucose monitoring using fluorescent hydrogel fibers. Heo YJ. et al. (2011). PNAS 108:13399-13403.
http://www.pnas.org/content/108/33/13399
17 Supramolecular nanostructures that mimic VEGF as a strategy for ischemic tissue repair. Webber MJ. et al. (2011). PNAS 108: 13438-13443.
http://www.pnas.org/content/108/33/13438
16 A fluorescent reporter, named traffic light, reads out whether repair of a DNA break occurs by nonhomologous end-joining or by homologous recombination. It should enable the identification of factors that affect repair pathway choice and thus improved approaches for genome engineering. Certo MT. et al. (2011). Nat Methods 8:671-676.
http://www.nature.com/nmeth/journal/v8/n8/abs/nmeth.1648.html
15 A large-scale method to measure absolute protein phosphorylation stoichiometries. Wu R. et al. (2011). Nat Methods 8: 677-683.
http://www.nature.com/nmeth/journal/v8/n8/abs/nmeth.1636.html
14 Two-photon polarization microscopy reveals protein structure and function. Lazar J. et al. (2011). Nat Methods 8:684-690.
http://www.nature.com/nmeth/journal/v8/n8/abs/nmeth.1643.html
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